Ginger (Zingiber officinale) extract mediated green synthesis of silver nanoparticles and evaluation of their antioxidant activity and potential catalytic reduction activities with Direct Blue 15 or Direct Orange 26

The green synthesis of silver nanoparticles (AgNPs) using a water extract of Ginger (Zingiber officinale) root by microwave irradiation and its antibacterial activities have been reported. However, AgNPs prepared from different parts of ginger root water or ethanol extract by ultrasound synthesis and their antioxidant activity and whether the biogenic could be used to catalyze the reduction of hazardous dye are unknown. This study concentrated on the facile green synthesis of AgNPs prepared from different parts (unpeeled ginger, peeled ginger, and ginger peel) of ginger root water or ethanol extract by the ultrasound-assisted method. We studied their antioxidant activity and catalytic degradation of hazardous dye Direct Orange 26 (DO26) and Direct Blue 15 (DB15). The surface plasmon resonance (SPR) peak of AgNPs was at 428–443 nm. The biogenic AgNPs were approximately 2 nm in size with a regular spherical shape identified from TEM analysis. The ethanol extracts of dried unpeeled ginger and peeled ginger, fresh peeled ginger and ginger peel. The Z. officinale AgNPs synthesized by dried unpeeled ginger ethanol extract showed the best antioxidant activity. Their scavenging activities were significantly better than BHT (p <0.05). The different parts of ginger extracts showed no catalytic degradation activities of DB15 and DO26. Still, the synthesized Z. officinale AgNPs exhibited good catalytic degradation activities, while their ability to catalytic degradation to DB15 was better than DO26. In the additive ratio of 3 mL DB15, 0.1 mL NaBH4 and 0.1 mL AgNPs, the degradation rates of DB15 (or DO26) at 15 min, 30 min and 60 min were only 1.8% (0.9%), 2.8% (1.4%) and 3.5% (1.6%) in the absence of AgNPs. When adding Z. officinale AgNPs prepared from dried ginger peel ethanol extract or fresh ginger peel water extract, the degradation rates of DB15 sharply increased to 97% and 93% after 30 min, respectively. In conclusion, ginger extract has good antioxidant properties. Z. officinale AgNPs biosynthesis from ginger extract exhibit excellent catalytic degradation activities, especially for the ginger peel extract. They have application value in the treatment of textile effluents and provide a new idea and method for the comprehensive development and utilization of ginger resources.

Introduction Silver nanoparticles (AgNPs) have received widespread attention due to their excellent properties like good photoelectrochemical activity, antimicrobial activity, anti-biofilms, electrical conductivity, and enzymatic activity [1][2][3][4][5]. The traditional preparation methods of AgNPs mainly include the physical and chemical methods [6][7][8][9]. As the potent reducing and capping agents frequently used in conventional methods are toxic to humans and the environment, green synthesis of AgNPs by a simple and eco-friendly method has gained more attention among researchers.
Biosynthesis has been preferred for synthesizing AgNPs to conventional methods using harmful reducing agents such as sodium borohydride, trisodium citrate, free aldehyde, ketone groups, and organic substances [10]. The biosynthesis of AgNPs using plant extracts instead of synthetic chemical reagents as the reducing, capping and stabilizing agents is one of the most commonly used green methods. Diverse plants such as Givotia moluccana [11], Rhodiola rosea [10,12], Corylus avellana [2], Oyster mushroom [13], Guettarda speciosa [14], Hagenia abyssinica [15], Syzygium cumini [16], Cassytha filiformis [17], Bryophyllum pinnatum [18], and Acacia cyanophylla [19] have been successfully used to synthesize AgNPs. This approach has the advantages of being cost-effective, environmentally friendly, non-toxic and large-scale production of AgNPs. In addition, the AgNPs produced by plant extract could remain stable even after prolonged storage since it prevents the molecules from accumulating.
Azo dyes are synthetic dyes that generally contain one or more azo bonds (-N = N-) in their chemical structures [20]. Azo dyes are frequently used in printing and dyeing, textile, paper making, pharmaceutical and other industries. Due to the structural stability and high chromaticity, azo dyes are resistant to degradation. The discharge of textile effluent could cause serious environmental problems. Less than 50% of dyes used are not being fixed in the products and tend to be discharged into the environment with the effluent, especially azo dyes. They threaten the health of humans and eco-systems [21,22]. Generally, the traditional physicochemical treatments of textile effluents are high-cost and may cause secondary pollution [23]. Therefore, there is an urgent need to develop more cost-effective, high-efficiency, and ecofriendly bioremediation technologies for textile effluents.
China is a major producer of ginger in the world. In 2020, the annual output of ginger in China reached 9.19 million tons, accounting for about 45% of the world's total output. Gingerol is a general name for spicy ingredients in ginger, composed of gingerol, shogaol, zingerone and other substances. It is also the main functional factor of ginger with various pharmacological effects. Gingerol has a variety of pharmacological functions, including scavenging free radicals, anti-oxidation, anti-mutation, anti-tumor, antibacterial, anti-viral and immune regulation, and is widely used in seasoning food, medicine, cosmetics and other industries [24,25].
Ginger peels are typically discarded during processing, resulting in a waste of resources. Thus, further exploring ginger peel usage, broadening the application value and scope, and promoting ginger's comprehensive utilization deserve investigation. The green synthesis of AgNPs using a water extract of whole ginger (Zingiber officinale) root by microwave irradiation and its antibacterial activities have been reported [26][27][28]. Ultrasound-assisted extraction uses the cavity effect and mechanical effect generated by ultrasonic waves to promote the movement of medium molecules, improve their movement speed and increase the penetration of the medium to promote the extraction of active components in the organism. In addition, it has the advantages of short time, high yield and low yield of by-products [29,30]. However, AgNPs prepared from different parts of ginger root water or ethanol extract by ultrasound synthesis and their antioxidant activity and whether the biogenic could be used to catalyze the reduction of hazardous dye are unknown. Therefore, this study concentrated on the facile green synthesis of AgNPs using ethanol or water extracts prepared from different parts (unpeeled ginger, peeled ginger, and ginger peel) of ginger roots by an ultrasound-assisted method. In addition, we studied their antioxidant activity and catalytic degradation of hazardous dye Direct Orange 26 (DO26) and Direct Blue 15 (DB15).
Preparation of fresh and dry root samples of Z. officinale. The fresh roots of Z. officinale were purchased from a local supermarket supplied by Shengjie Co. (Weifang, Shandong, China, 35.41˚N and 108.10˚E). Unpeeled ginger, peeled ginger, and ginger peel were taken separately and were cut off approximately 0.008 cm 3 . The various gingers were named fresh unpeeled ginger, fresh peeled ginger, and fresh ginger peel. They were sealed into bags separately and were stored at -4˚C.
The obtained fresh unpeeled ginger, fresh peeled ginger, and fresh ginger peel were dried by hot air at 80˚C for about 6-8 h, ground into powder (FW177 mill, Taisite Co., Tianjin, China) and screened by a 40-mesh sieve [10]. The obtained powders were named dry unpeeled ginger, dry peeled ginger, and dry ginger peel, respectively. They were sealed into bags separately and were stored at -20˚C.
Preparation of extract from fresh or dry root of Z. officinale. Samples of fresh unpeeled ginger, peeled fresh ginger, and fresh ginger peel (5.0 g) were separately ultrasonically extracted with 50 mL of deionized water for 30 min at 60˚C with a constant power of 200 W and a fixed frequency of 40 kHz (KQ500E Ultrasonic Cleaner, Kunshan, China). They were centrifuged at 3500 rpm for 10 min (5810R Centrifuge, Eppendorf Co., Hamburger, Germany), and the supernatant (water phase) was transferred into a vial [31]. The water phase was then collected and diluted with deionized water to 50 mL. The obtained solution was named fresh unpeeled ginger water extract, fresh peeled ginger water extract, and fresh ginger peel water extract were stored at -4˚C, respectively. The ethanol extract of unpeeled fresh ginger, ethanol extracts of peeled fresh ginger and fresh ginger peel were prepared by a similar method, except the extracting solvent was replaced with 70% ethanol. All experiments were repeated three times.
They were centrifuged at 3500 rpm for 10 min (5810R Centrifuge, Eppendorf Co., Hamburger, Germany), and the supernatant (water phase) was transferred into a vial [31]. The water phase was then collected and diluted with deionized water to 50 mL. The obtained solutions were named dry unpeeled ginger water extract, dry peeled ginger water extract, and dry ginger peel water extract. The ethanol extracts of unpeeled dry ginger, peeled dry ginger, and dry ginger peel were prepared using a similar method, except the extracting solvent was replaced with 70% ethanol. All experiments were repeated three times.

Green synthesis of colloidal AgNPs using Z. officinale root extract
The green synthesis of colloidal AgNPs using Z. officinale was conducted using a previously reported method with minor modifications [10,26]. First, one mL of the extracts of Z. officinale roots was added to 20 mL of silver nitrate solution (1.0 mmol/L). Then, the mixtures were separately ultrasound for 30 min at 60˚C with a constant power of 200 W and a fixed frequency of 40 kHz (KQ500E Ultrasonic Cleaner, Kunshan, China).

Characterization of AgNPs
Ultraviolet-visible spectrophotometry. An ultraviolet-visible spectrophotometer (UVvis) (Mapada UV-3200, Shanghai) was used to confirm metal ions' reduction into metal nanoparticles. For UV-visible spectroscopy studies, the synthesized colloidal AgNPs were scanned directly. The surface plasmon resonance peak was observed between 400 and 500 nm in the visible region. In addition, the absorbance was measured in the wavelength range between 200 and 800 nm [10,32].
HR-TEM. Transmission electron microscopy (TEM) was performed on a Tecnai G2F20STWIN system (Thermo Fisher, Amsterdam, Netherlands) to evaluate the size and morphology at an accelerating voltage of 200 kV [33].
XRD. The prepared colloidal AgNPs were centrifuged at 10000 rpm /min for 10 min and then precipitated and dried at 60˚C to obtain black solid particles. The solid particles were characterized by a D/max-2200 X-ray diffractometer (Rigku, Tokyo, Japan) using Cu Kα radiation (λ = 0.154 nm) [32,33].

Evaluation of the antioxidant activity of AgNPs
The antioxidant activity of AgNPs was determined by the DPPH radical scavenging method with minor modifications [10,34]. Three mL of DPPH-ethanol solution (40 mg/L) and 200 μL of Z. officinale root extract or Z. officinale AgNPs, and BHT at the concentration of 1×10 −3 mol/L were added into the centrifuge tube and then incubated for 1 h with oscillation at room temperature in the dark. The absorbance of the supernatant at 517 nm was determined. Ethanol solution was used as blank control, and BHT was used as a standard antioxidant. Antioxidant activity was measured by inhibition rate I % = [1-(A 0 -A 1 )/A 2 ] × 100. In the formula, I is the inhibition ratio, A 0 is the absorbance of the sample solution, A 1 is the absorbance of the ethanol-sample solution, and A 2 is the absorbance of the DPPH-ethanol solution. All experiments were repeated three times.

Evaluation of the catalytic activity of Z. officinale root extract or Z. officinale AgNPs
The catalytic activity of AgNPs was determined by a published method with minor modifications [10,32,35]. The catalytic activity of Z. officinale root extract or synthesized Z. officinale AgNPs was determined based on the reductive degradation of DO26 and DB15 in the presence of NaBH 4 . Three milliliters of DO26 or DB15 (50 mg/L) were mixed with 0.1 mL NaBH 4 (0.1 mol/L) and 0.1 mL Z. officinale root extract or synthesized Z. officinale AgNPs. The performance was monitored by recording UV-vis spectra variation over time (0, 3,6,9,12,15,18,21,24,27,30,40,50, and 60 min). The control was also conducted without extract or AgNPs. The degradation rates for 15, 30, and 60 min were calculated. The degradation rate calculation formula was (1-A 1 /A 0 ) � 100, where A 0 and A 1 are the absorbance values at a wavelength of 603 nm (for DB15)/495 nm (for DO26) at 0 min, 15 min (or 30 min and 60 min), respectively. A pseudo-first-order reaction was assumed for the concentration of DO26 or DB15 to evaluate the reaction kinetics. The integrated form of first-order reaction is expressed as follows: ln A t / A 0 = -kt. A t is the absorbance at time t, A 0 is the absorbance at time 0, and k is the rate constant. All experiments were repeated three times.

Statistical analysis
Data are expressed as the mean values ± standard deviation (SD) and were analyzed using one-way ANOVA. Duncan's was used to determine significant differences between treatments. All statistical procedures were conducted using SPSS 22.0 for Windows. A significance level of p<0.05 was used.

Spectroscopic and structural characterization of extract and synthesized AgNPs
The extracts from different parts of fresh and dried ginger were nearly colorless to varying degrees of yellow transparent solutions. However, the color of ethanol extract was darker than that of water extract. After adding the extract of Z. officinale root to the colorless solution of silver nitrate, a visible color change was noticed from transparent to brownish red colloidal solution. The color of Z. officinale AgNPs prepared using ethanol extract was darker than water extract (Fig 1). The color change indicates the formation of colloidal silver particles, confirmed by ultraviolet absorption spectrum, TEM and XRD (Figs 2-4). Fig 2 shows the absence of an SPR peak for the different ginger root extracts, but an SPR peak appeared around 428-443 nm. The SPR peak absorbance and peak width of Z. officinale AgNPs synthesized using various methods differed greatly. For example, the SPR peak absorbance of Z. officinale AgNPs synthesized using ethanol extract was higher (p <0.05) than water extract with smaller peak width. The SPR peak absorbance of Z. officinale AgNPs synthesized using ethanol extract of dry ginger was higher (p <0.05) than ethanol extract of fresh ginger with smaller peak width. The SPR peak absorbance of Z. officinale AgNPs synthesized using water extract of fresh ginger was higher (p <0.05) than water extract of fresh ginger with a smaller peak width. TEM results show that the AgNPs synthesized using water extract of fresh ginger peel had uniform particle size distribution, which was regular and spherical, and the particle size was about 2 nm. But the AgNPs synthesized using ethanol extract of fresh unpeeled ginger, and the AgNPs synthesized using water extract of the dry unpeeled ginger show a diameter of about 20 nm with a cluster distribution (Fig 3). XRD results show that there were three significant diffraction peaks at 2θ angles 38.116˚, 44.277å nd 64.426˚, indicating that silver nanoparticles were successfully synthesized (Fig 4). The colloidal Z. officinale AgNPs synthesized from different methods show good stability, they could be maintained for several months.

Evaluation of the antioxidant activity of Z. officinale root extract
Ginger extracts prepared by different methods significantly affected DPPH scavenging activity (p <0.05). For both fresh ginger and dry ginger, the antioxidant activity of ethanol extract from different parts of ginger was better than that of water extract (p <0.05). The ethanol extracts of unpeeled dry ginger, peeled dry ginger, peeled fresh ginger, and fresh ginger peel showed the best antioxidant activity. Their scavenging activities against DPPH were 65.6%, 63.0%, 57.6% and 54.3%, respectively, which were better (p <0.05) than that of BHT (42.3%). Nevertheless, the DPPH scavenging activity of water extract of unpeeled fresh ginger and ginger peel was lower (p <0.05) than that of BHT (Table 1). Ginger extract and its constituent components (such as 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol) have been shown to have good antioxidant properties. This observation may be related to their hydroxyl groups and appropriate solubilizing side chains [36,37]. In this study,

Evaluation of the antioxidant activity of synthesized Z. officinale AgNPs
The DPPH scavenging activity of Z. officinale AgNPs was lower than that of ginger root extract. Z. officinale AgNPs synthesized by different methods affected DPPH scavenging activity (p <0.05). For both fresh ginger and dry ginger, the antioxidant activity of Z. officinale AgNPs prepared using ethanol extract was better than that prepared using water extract (p <0.05). The Z. officinale AgNPs synthesized using ethanol extract of dry peeled ginger showed the best antioxidant activity, and its scavenging activities against DPPH was 64.9%, which was better (p <0.05) than that of BHT (42.3%). Z. officinale AgNPs synthesized using ethanol extract of fresh ginger peel also showed good antioxidant activity. Its scavenging activity against DPPH was 36.2%, and there was no difference (p >0.05) compared to BHT ( Table 2). AgNPs colloids synthesized using R. rosea rhizome extract exhibited good antioxidant activity [10]. Its excellent antioxidant activity was attributed to antioxidant polyphenolic compounds such as phenylpropanoids, flavonoids and cinnamyl alcohol. In this study, Z. officinale AgNPs synthesized using ethanol extract of peeled dry ginger exhibited        (Figs 5 and 6). The appearance of a new absorption peak was due to the addition of Z. officinale AgNPs, which indicates the presence of AgNPs. In this research, the additive ratio was 3 mL DB15, 0.1 mL NaBH 4 and 0.1 mL AgNPs. Generally, the degradation rate increased, and the degradation rate constant decreased with the increasing reaction time. In addition, the catalytic degradation effect of AgNPs prepared using the dry ginger extract was higher (p<0.05) than AgNPs prepared using fresh ginger extract. And AgNPs prepared using ethanol extract of ginger were better (p<0.05) than water extract (Figs 5 and 6).
The catalytic degradation of AgNPs prepared from ethanol extract of unpeeled dry ginger showed the best. The degradation rate of DB15 at 3, 6, 9, 12, 15, 18, 21, 24, and 27 min gradually increased from 28%, to 45%, 83%, 86%, 87%, 92%, 94%, 96%, and 97%, respectively. The degradation rate constants at 15 min and 30 min were higher than others Z. officinale AgNPs (Fig 6 and Table 3). The AgNPs prepared from water or ethanol extract of fresh ginger peel, and ethanol extract of the dry ginger (unpeeled ginger, peeled ginger and ginger peel) showed excellent catalytic activity of DB15 in a short time. For example, the degradation rates of DB15 could reach 86% after a reaction of 15 min (Figs 5 and 6 and Table 3). Furthermore, the AgNPs prepared from water extract of fresh ginger peel had higher (p<0.05) catalytic degradation of DB15 than dried ginger peel water extract (Figs 5 and 6 and Table 3).
For AgNPs prepared from fresh ginger extract, the catalytic degradation activity of ginger peel was better (p<0.05) than unpeeled and peeled ginger extract. Among them, AgNPs prepared from water and ethanol extract of ginger peel showed the best catalytic degradation activity for DB15; the degradation rate reached 83% after 15 min, and their catalytic degradation activity did not differ (p>0.05) ( Table 3). While the AgNPs prepared using ethanol extract of unpeeled ginger exhibited a minimum catalytic activity (Fig 5 and Table 3).
For AgNPs prepared using dry ginger, the catalytic degradation activity from ethanol extract was better (p<0.05) than the water extract. While the catalytic degradation activity of AgNPs prepared from three ethanol extracts of ginger all showed excellent catalytic activity in DB15 in a short time, such as 15 min, the degradation rate reached the maximum of 85%. However, no difference (p>0.05) was observed among them (Table 3). In contrast, the catalytic degradation activity of the AgNPs prepared from different water extracts showed a significant difference (p<0.05), and the catalytic activities were in order of peeled ginger, unpeeled ginger and ginger peel. The catalytic activity of AgNPs synthesized using water extract of dry ginger was lower (p<0.05) in 15 min, but the degradation rate increased with the extension of time. For example, after a reaction of 60 min, the degradation rate of DB15 for AgNPs prepared from ethanol extract of unpeeled dry ginger reached a maximum of 98% (Fig 6 and Table 3).
The comparisons of degradation parameters for DB15 by AgNPs synthesized from plant extracts reported in the references are listed in Table 5. Compared to R. rosea AgNPs and C-AgNPs (chemically synthesized by reducing NaBH 4 ), the amount of DB15 was lower, and the amount of NaBH 4 and AgNPs was higher. Thus, the catalytic degradation ability of R. rosea AgNPs and C-AgNPs was much lower than synthesized Z. officinale AgNPs in this study. Compared to the tea polyphenol AgNPs [38], Z. officinale AgNPs exhibited excellent catalytic activity in a much shorter time (15min VS 80min) under the same ratio. Compared to the previous report, the AgNPs synthesized from Z. officinale showed higher catalytic degradation activity of DB15.
The catalytic reduction of DB15 and DO26 was usually explained in terms of the Langmuir-Hinshelwood model [32,39]. Electron transfer plays a crucial role in the catalytic effect of AgNPs; that is, the AgNPs play a role in electron transfer between NaBH 4 and dye, realizing the transfer of electrons between the provider (NaBH 4 ) and the recipient (dye). When there is  no catalyst, there is an electron transfer barrier between the reducing agent (NaBH 4 ) and the dye, so the reduction could not be effective. Based on this, in catalytic reduction, NaBH 4 and dye should be adsorbed on the surface of the AgNPs catalyst as much as possible to achieve more efficient electron transfer and reduce the degradation of dye. At the same time, the surface adsorption capacity of AgNPs is closely related to their particle size distribution, and particles with small particle size distribution tend to have stronger adsorption capacity. Therefore, the excellent catalytic activity of Z. officinale AgNPs in this study may be attributed to its smaller particle size. Catalytic activity of ginger extract on the reduction of Direct Blue 15 by NaBH 4 . As shown in Figs 7 and 8, the water and ethanol extract from different parts of fresh and dry ginger showed no catalytic degradation activities of DB15.

Catalytic activity of colloidal AgNPs and ginger extract on the reduction of Direct Orange 26 by NaBH 4
Catalytic activity of colloidal Z. officinale AgNPs synthesized from ginger extract on the reduction of Direct Orange 26 by NaBH 4 . The synthesized Z. officinale AgNPs colloids were also directly used to compare the catalytic activities for the degradation of DO26 without further centrifugation. The maximum absorption of DO26 was at 494 nm, so the absorbance value at 494 nm was chosen to monitor the degradation of the azo dye. Without the addition of colloidal Z. officinale AgNPs, the characteristic peak of DO26 did not change (p>0.05) with the extension of time. However, the characteristic peak of DO26 decreased (p<0.05) continuously with the addition of Z. officinale AgNPs, which indicated that Z. officinale AgNPs had good catalytic abilities in the reduction of DO26 degradation (Figs 9 and 10). With the addition of Z. officinale AgNPs, a new peak near 430 nm appeared. The appearance of a new absorption peak was due to adding Z. officinale AgNPs, which indicates the presence of AgNPs.
In this research, the addition ratio of DO26 (50 mg/L), NaBH 4 (0.1 mol/L) and AgNPs was V (DO26): V(NaBH 4 ): V(AgNPs) = 3:0.1:0.1. In general, the catalytic degradation activity of the synthesized Z. officinale AgNPs for DO26 was lower than that for DB15 under the same conditions. The catalytic degradation activity of Z. officinale AgNPs prepared by ginger peel extract for DO26 was higher (p<0.05) than that of unpeeled ginger and peeled ginger. Among them, the catalytic degradation activity of Z. officinale AgNPs prepared from fresh ginger peel was superior to dry ginger peel, and the ethanol extract of ginger peel was better (p<0.05) than that of water extract of ginger peel (Figs 9 and 10 and Table 4).
The degradation rate increased, and the degradation rate constant decreased with the increasing reaction time. Z. officinale AgNPs prepared from the ethanol extract of fresh ginger peel showed the maximum catalytic degradation activity for DO26, and the degradation rate reached 72%. The Z. officinale AgNPs prepared from the ethanol extract of dry ginger peel also showed good catalytic degradation activity for DO26, but the degradation rate only reached 41% (Figs 9 and 10 and Table 4).
The comparisons of degradation parameters for DO26 by AgNPs synthesized from plant extracts reported in the references are listed in Table 5. Compared to R. rosea AgNPs and C-AgNPs [10], the amount of DO26 was lower. On the other hand, the amount of NaBH 4 and AgNPs was higher. Thus the catalytic degradation ability of R. rosea AgNPs and C-AgNPs was much higher than synthesized Z. officinale AgNPs in this study. Compared to the Eucommia ulmoides AgNPs [40] and grape seed AgNPs [35] under the same ratio, the AgNPs synthesized from Z. officinale showed poor catalytic degradation activity for DO26 ( Table 5). The Z. officinale AgNPs prepared using ginger extract have some catalytic degradation activity for DO26 but are lower than the previous report. Ginger extract has a smaller particle size, but its catalytic activity for DB15 and DO26 varied. However, the specific mechanism needs to be further studied.
Catalytic activity of ginger extract on the reduction of Direct Orange 26 by NaBH 4 . As shown in Figs 11 and 12, the water and ethanol extracts from different parts of fresh and dried ginger had no catalytic degradation activities of DO26.

Conclusions
This study concentrated on the facile green synthesis of AgNPs using ethanol or water extracts from different parts (unpeeled ginger, peeled ginger, and ginger peel) of the ginger root by the ultrasound-assisted method. We studied their antioxidant activity and catalytic degradation of hazardous dye DB15 and DO26. The surface plasmon resonance (SPR) peak of AgNPs was at 428-443 nm. The colloidal Z. officinale AgNPs synthesized from different ginger extract show good stability. The biogenic Z. officinale AgNPs synthesized using water extract of fresh ginger peel were approximately 2 nm in size with a regular spherical shape identified from TEM analysis. But the AgNPs synthesized using ethanol extract of fresh unpeeled ginger, or water extract of the dry unpeeled ginger show a diameter of about 20 nm with a cluster distribution. The ethanol extracts of unpeeled dry ginger and peeled dry ginger, peeled fresh ginger, fresh ginger peel, and the Z. officinale AgNPs synthesized by ethanol extract of unpeeled dry ginger showed the best antioxidant activity. Their scavenging activities were significantly better than BHT (p <0.05). The different parts of ginger extracts showed no catalytic degradation activities of DB15 and DO26. Still, the synthesized Z. officinale AgNPs exhibited good catalytic degradation activities, while their ability to catalytic degradation to DB15 was better than DO26. In the additive ratio of 3 mL DB15, 0.1 mL NaBH 4 and 0.1 mL AgNPs, the degradation rates of DB15 (or DO26) at 15 min, 30 min and 60 min were only 1.8% (0.9%), 2.8% (1.4%) and 3.5% (1.6%) in the absence of AgNPs. When adding Z. officinale AgNPs prepared from ethanol extract of unpeeled dry ginger or water extract of fresh ginger peel, the degradation rates of DB15 sharply increased to 97% and 93% after 30 min, respectively. In conclusion, ginger extract has good antioxidant properties. Z. officinale AgNPs biosynthesis from ginger extract exhibit excellent catalytic degradation activities, especially for the ginger peel extract. They have application value in the treatment of textile effluents and provide a new idea and method for the comprehensive development and utilization of ginger resources.